Synthesis and Characterisation of Proteins Profile of “<i>Streptomyces coelicolor</i>” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation

Teresa Matoso Manguangua Victor; Miguel Filho; Jose Maria Samuco; Paul Kamps; Steve Bull; Jarka Glassey; Alan Claude Ward

doi:doi:10.11648/j.ajcbe.20220602.12

Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation

Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, Jarka Glassey, Alan Claude Ward

Published in American Journal of Chemical and Biochemical Engineering (Volume 6, Issue 2)

Received: 11 September 2022 Accepted: 26 September 2022 Published: 18 October 2022

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Abstract

In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 6, Issue 2)
DOI	10.11648/j.ajcbe.20220602.12
Page(s)	51-67
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

2-VynilPyridine (2VP), PolyHIPE Polymer (PHP), Streptomyces coelicolor A3 (2), MALDI-TOF/MS, Protein

References

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Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, et al. (2022). Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. American Journal of Chemical and Biochemical Engineering, 6(2), 51-67. https://doi.org/10.11648/j.ajcbe.20220602.12

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Teresa Matoso Manguangua Victor; Miguel Filho; Jose Maria Samuco; Paul Kamps; Steve Bull, et al. Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. Am. J. Chem. Biochem. Eng. 2022, 6(2), 51-67. doi: 10.11648/j.ajcbe.20220602.12

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Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, et al. Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. Am J Chem Biochem Eng. 2022;6(2):51-67. doi: 10.11648/j.ajcbe.20220602.12

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@article{10.11648/j.ajcbe.20220602.12,
  author = {Teresa Matoso Manguangua Victor and Miguel Filho and Jose Maria Samuco and Paul Kamps and Steve Bull and Jarka Glassey and Alan Claude Ward},
  title = {Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {6},
  number = {2},
  pages = {51-67},
  doi = {10.11648/j.ajcbe.20220602.12},
  url = {https://doi.org/10.11648/j.ajcbe.20220602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20220602.12},
  abstract = {In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.},
 year = {2022}
}

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TY  - JOUR
T1  - Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation
AU  - Teresa Matoso Manguangua Victor
AU  - Miguel Filho
AU  - Jose Maria Samuco
AU  - Paul Kamps
AU  - Steve Bull
AU  - Jarka Glassey
AU  - Alan Claude Ward
Y1  - 2022/10/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajcbe.20220602.12
DO  - 10.11648/j.ajcbe.20220602.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 51
EP  - 67
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20220602.12
AB  - In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.
VL  - 6
IS  - 2
ER  -

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Author Information

Teresa Matoso Manguangua Victor

Department of Engineering and Technology, Higher Polytechnic Institute of Technology and Science (ISPTEC), Luanda, Angola
Miguel Filho

Department of Engineering and Technology, Higher Polytechnic Institute of Technology and Science (ISPTEC), Luanda, Angola
Jose Maria Samuco

Department of Engineering and Technology, Higher Polytechnic Institute of Technology and Science (ISPTEC), Luanda, Angola
Paul Kamps

Department of Research and Development, Nutriss Inc, Wellington, Florida, USA
Steve Bull

School of Chemical Engineering and Advanced Materials, University of Newcastle Upon Tyne, Newcastle Upon Tyne, UK
Jarka Glassey

School of Chemical Engineering and Advanced Materials, University of Newcastle Upon Tyne, Newcastle Upon Tyne, UK
Alan Claude Ward

School of Biology and Psychology, University of Newcastle upon Tyne, Newcastle Upon Tyne, UK

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Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, et al. (2022). Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. American Journal of Chemical and Biochemical Engineering, 6(2), 51-67. https://doi.org/10.11648/j.ajcbe.20220602.12

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Teresa Matoso Manguangua Victor; Miguel Filho; Jose Maria Samuco; Paul Kamps; Steve Bull, et al. Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. Am. J. Chem. Biochem. Eng. 2022, 6(2), 51-67. doi: 10.11648/j.ajcbe.20220602.12

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Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, et al. Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. Am J Chem Biochem Eng. 2022;6(2):51-67. doi: 10.11648/j.ajcbe.20220602.12

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@article{10.11648/j.ajcbe.20220602.12,
  author = {Teresa Matoso Manguangua Victor and Miguel Filho and Jose Maria Samuco and Paul Kamps and Steve Bull and Jarka Glassey and Alan Claude Ward},
  title = {Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {6},
  number = {2},
  pages = {51-67},
  doi = {10.11648/j.ajcbe.20220602.12},
  url = {https://doi.org/10.11648/j.ajcbe.20220602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20220602.12},
  abstract = {In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.},
 year = {2022}
}

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TY  - JOUR
T1  - Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation
AU  - Teresa Matoso Manguangua Victor
AU  - Miguel Filho
AU  - Jose Maria Samuco
AU  - Paul Kamps
AU  - Steve Bull
AU  - Jarka Glassey
AU  - Alan Claude Ward
Y1  - 2022/10/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajcbe.20220602.12
DO  - 10.11648/j.ajcbe.20220602.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 51
EP  - 67
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20220602.12
AB  - In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.
VL  - 6
IS  - 2
ER  -

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